Affinity pulldown protocols for active RhoA, B, C, Rac, Cdc42 and RhoG.
Nuclear Rho and GEF pulldowns
Affinity pulldowns for active GTPases and RhoGEFs from nuclear extracts
Our tried and true IF protocol. Originally obtained from Elizabeth Sztul's lab. However I can't say for sure if it was developed in her lab or not. It works great regardless.
Calcium Phosphate Transfection
A great (and very cheap) way to transfect DNA into easy to transfect cells. Especiall useful for protein-protein interaction studies. This protocol comes courtesy of the great Etienne Boulter.