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Our tried and true IF protocol. Originally obtained from Elizabeth Sztul's lab. However I can't say for sure if it was developed in her lab or not. It works great regardless.
Calcium Phosphate Transfection
A great (and very cheap) way to transfect DNA into easy to transfect cells. Especiall useful for protein-protein interaction studies. This protocol comes courtesy of the great Etienne Boulter.
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